In Designing the Experiment, the Researchers Used Which Type of 32p Labeled ATP?

Question:

In designing the experiment, the researchers used which type of 32p labeled ATP?

Answer:

Y32P-ATP

Since the phosphoryl transfer from kinases Stems in the Y-phosphate of ATP.
Consequently, the experimentation should demand Y32P-ATP


Question:

What atom is the site of covalent attachment of AMC to the model tetrapeptide used in the studies?

Answer:

I

Since AMC Has Been attached to the peptide Around the carboxyl side.
This implies an amide linkage between the N receptor in AMC can be used to covalently attach the fluorophore into the peptide


Question:

Absorption of UV light by organic molecules always results in what process?

Answer:

Excitation of bound electrons


Question:

Four organic compounds: 2-butanone, n-pentane, propanoic acid, and n-butanol, present as a mixture, are separated by column chromatography using silica gel with benzene as the eluent. What is the expected order of elution of these four organic compounds from first to last?

Answer:

The four chemicals possess similar molecular weights, so the sequence of elution will rely on the polarity of the molecule. Considering that silica gel serves as the stationary stage for its experimentation, raising the polarity of this eluting molecule increases its affinity for the stationary phase and increase the elution period (diminished Rf)


Question:

The half life of a radioactive material is:

Answer:

The time it takes to Get half of All of the radioactive nuclei to decay into their daughter nuclei

–Since the half life of a radioactive Substance is Described as the time it takes for half of All of the radioactive nuclei to decay into their daughter nuclei, Which Might or Might Not Be Freezing


Question:

Which amino acid will contribute to the CD signal in the far UV region, but NOT the near UV region when part of a fully folded protein?

Answer:

Ala

All chiral nonaromatic amino acids will contribute solely to the CD signal in the far UV region

It’s in the passage!! read and trust your gut!!


Question:

Based on the relative energy of the absorbed electromagnetic radiation, which absorber, a peptide bond or an aromatic side chain, exhibits an electronic excited state that is closer in energy to the ground state?

Answer:

An aromatic side chain; the absorbed photon energy is Reduced

–Since aromatic side chains consume in the near UV region of the electromagnetic spectrum, that includes more wavelengths, and thus lower energy compared to peptide bonds. Since the power of this photon matches with the energy difference between the ground and the excited condition, this implies that the aromatic side-chain has more tightly spaced energy levels

Herean”electron excitation say nearer to the ground say” is only a fancy way of stating energy. To find out that (aromatic side chain or peptide bond) may get lower energy let us use the formula E = hf, which informs us that the power of electromagnetic radiation is directly due to its frequency.

In the passage, we realize that the aromatic side-chain consumes electromagnetic radiation at the 250-290nm array whereas the peptide bond consumes from the 190-250nm variety. Bear in mind, the wavelength is INVERSELY proportional to frequency, therefore that the longer wavelength light consumed by aromatic side chains are going to have reduced frequency. Considering that our equation and with no calculations we could say that aromatic side chains may consume less energy and possess an electron excitation condition nearer to the ground condition.

In Designing the Experiment, the Researchers Used Which Type of 32p Labeled ATP?

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